Optimizing an in vitro model for head and neck squamous cell carcinoma including multicellular spheroids of cancer and stroma cells
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Julkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty.
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Head and neck cancers are the 6th most common cancers worldwide. 90% of them are head and neck squamous cell carcinoma (HNSCC). Currently due to the lack of effective screening strategies and limited treatment options, HNSCC patients have poor prognosis and the five-year survival rate averaging around 50% globally. Therefore, new prognostic and predictive biomarkers and advanced disease models are urgently needed to facilitate early diagnosis and the development of more efficient targeted therapies. This study explores the possibility of creating and combining head and neck cancer 3D co-culture tumor spheroids, with a millifluidic device platform to better replicate the tumor microenvironment.
This study includes optimizing experimental conditions, such as the cell number for spheroid culturing and coculturing the spheroids using a commercial human tongue squamous cell carcinoma cell line, cancer-associated fibroblasts and human adipose microvascular endothelial cells. The spheroids were formalin-fixed and embedded in paraffin for further examination using hematoxylin and eosin staining and immunofluorescence staining. Lastly a trial run of the millifluidic device was conducted.
Co-culture spheroids were successfully cultured with three different cell types. The spheroids exhibited distinct behavioral differences compared to tumor-cellonly spheroids, and systematic cell distribution could be distinguished. The incorporation of the spheroid with the chip model was successful. However, it was not effective for studying immune cell migration. Still the co-culture spheroid model better represented the tumor microenvironment and holds the potential to be developed and applied to study the disease characteristics and for drug testing.