SARS-CoV-2 seroprevalence and seroconversion in Finnish households with school-aged children between June 2020 and April 2022

Abstract

<p>Background<br></p><p>SARS-CoV-2 spreads efficiently in households with children. Here, we aimed to examine whether serological methods, in addition to PCR, can improve understanding of SARS-CoV-2 transmission within households.<br></p><p>Methods<br></p><p>Within the prospective birth-cohort study called the STEPS Study, 175 households with 376 children and 324 adults were prospectively followed for acute respiratory infections from June 2020 to April 2022. SARS-CoV-2 was tested by PCR and antigen tests. IgG antibodies against SARS-CoV-2 nucleoprotein (N) and spike (S) proteins were determined at recruitment and at two later timepoints to assess seroprevalence, and five weeks after a PCR-positive infection in the household to assess seroconversion. Secondary attack rates (SARs) based on seroconversion or PCR-positivity were calculated and compared among index and secondary cases.<br></p><p>Results<br></p><p>SARS-CoV-2 anti-N-IgG seroprevalence showed a minor increase from 0.5% at recruitment, starting in June 2020, to1.9% at the last serum collection. SARS-CoV-2 RNA was detected by PCR in altogether 334 (48%) participants. The SAR for the adult index cases was higher than the SAR for the child index cases (77% vs 48%; p = 0.0027). The SARs were similar for unvaccinated (0–1 vaccinations) and vaccinated (2–3 vaccinations) index cases (53% and 63%, respectively; p = 0.28), but the SAR for unvaccinated secondary cases was higher compared to the SAR for vaccinated secondary cases (83% vs 52%; p = 0.0067).<br></p><p>Conclusion<br></p><p>In this study, adults spread SARS-CoV-2 in households more efficiently than children. While the index case vaccination status showed no difference, unvaccinated household members were more prone to SARS-CoV-2 infection than vaccinated individuals.<br></p><p>Key points<br></p><p>A cohort of households with children was prospectively studied for SARS-CoV-2 transmission through repeated PCR, and serology was obtained 5 weeks post-infection. Adults transmitted the virus more effectively than children, and unvaccinated participants were more susceptible than vaccinated participants.<br></p><p>Keywords: children, COVID-19, household transmission, SARS-CoV-2, secondary attack rate, seroconversion, serology<br></p>