A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites

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dc.contributor.authorPihlajamaa Päivi
dc.contributor.authorKauko Otto
dc.contributor.authorSahu Biswajyoti
dc.contributor.authorKivioja Teemu
dc.contributor.authorTaipale Jussi
dc.contributor.organizationfi=Turun biotiedekeskus|en=Turku Bioscience Centre|
dc.contributor.organization-code1.2.246.10.2458963.20.18586209670
dc.converis.publication-id177252758
dc.converis.urlhttps://research.utu.fi/converis/portal/Publication/177252758
dc.date.accessioned2022-12-21T03:30:52Z
dc.date.available2022-12-21T03:30:52Z
dc.description.abstract<p>Here we describe a competitive genome editing method that measures the effect of mutations on molecular functions, based on precision CRISPR editing using template libraries with either the original or altered sequence, and a sequence tag, enabling direct comparison between original and mutated cells. Using the example of the MYC oncogene, we identify important transcriptional targets and show that E-box mutations at MYC target gene promoters reduce cellular fitness. A competitive CRISPR method discovers targets and phenotypic effects of the MYC oncogene.</p>
dc.format.pagerange197
dc.format.pagerange203
dc.identifier.eissn1546-1696
dc.identifier.jour-issn1087-0156
dc.identifier.olddbid190727
dc.identifier.oldhandle10024/173818
dc.identifier.urihttps://www.utupub.fi/handle/11111/32148
dc.identifier.urlhttps://www.nature.com/articles/s41587-022-01444-6
dc.identifier.urnURN:NBN:fi-fe2022121371230
dc.language.isoen
dc.okm.affiliatedauthorKauko, Otto
dc.okm.discipline1183 Plant biology, microbiology, virologyen_GB
dc.okm.discipline318 Medical biotechnologyen_GB
dc.okm.discipline1183 Kasvibiologia, mikrobiologia, virologiafi_FI
dc.okm.discipline318 Lääketieteen bioteknologiafi_FI
dc.okm.internationalcopublicationinternational co-publication
dc.okm.internationalityInternational publication
dc.okm.typeA1 ScientificArticle
dc.publisherNature Portfolio
dc.publisher.countryUnited Statesen_GB
dc.publisher.countryYhdysvallat (USA)fi_FI
dc.publisher.country-codeUS
dc.relation.doi10.1038/s41587-022-01444-6
dc.relation.ispartofjournalNature Biotechnology
dc.relation.volume41
dc.source.identifierhttps://www.utupub.fi/handle/10024/173818
dc.titleA competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites
dc.year.issued2023

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