Isolation of the B Cell Immune Synapse for Proteomic Analysis

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dc.contributor.authorCunha Diogo M
dc.contributor.authorHernández-Pérez Sara
dc.contributor.authorMattila Pieta K
dc.contributor.organizationfi=InFLAMES Lippulaiva|en=InFLAMES Flagship|
dc.contributor.organizationfi=MediCity|en=MediCity|
dc.contributor.organizationfi=Turun biotiedekeskus|en=Turku Bioscience Centre|
dc.contributor.organizationfi=biolääketieteen laitos|en=Institute of Biomedicine|
dc.contributor.organization-code1.2.246.10.2458963.20.18586209670
dc.contributor.organization-code1.2.246.10.2458963.20.68445910604
dc.contributor.organization-code1.2.246.10.2458963.20.77952289591
dc.converis.publication-id179763891
dc.converis.urlhttps://research.utu.fi/converis/portal/Publication/179763891
dc.date.accessioned2025-08-28T03:38:28Z
dc.date.available2025-08-28T03:38:28Z
dc.description.abstractRecent technical developments have fueled increasing utilization of proteomics to gain new insights into various aspects of cellular behavior. In this chapter, we describe a method to specifically isolate immune synapses from mouse primary B cells. The method utilizes antibody-coated magnetic beads to induce the formation of the immune synapses and describes a protocol for the extraction of the cell-bead adhesions for mass spectrometry analysis. Finally, this method enables unveiling the large-scale protein content of the immune synapse.
dc.format.pagerange393
dc.format.pagerange408
dc.identifier.eisbn978-1-0716-3135-5
dc.identifier.isbn978-1-0716-3134-8
dc.identifier.issn1064-3745
dc.identifier.olddbid210939
dc.identifier.oldhandle10024/193966
dc.identifier.urihttps://www.utupub.fi/handle/11111/56705
dc.identifier.urnURN:NBN:fi-fe2025082788753
dc.language.isoen
dc.okm.affiliatedauthorCunha, Diogo
dc.okm.affiliatedauthorHernandez Perez, Sara
dc.okm.affiliatedauthorMattila, Pieta
dc.okm.affiliatedauthorDataimport, MediCity
dc.okm.discipline3111 Biomedicineen_GB
dc.okm.discipline318 Medical biotechnologyen_GB
dc.okm.discipline3111 Biolääketieteetfi_FI
dc.okm.discipline318 Lääketieteen bioteknologiafi_FI
dc.okm.internationalcopublicationnot an international co-publication
dc.okm.internationalityInternational publication
dc.okm.typeA3 Book
dc.publisherHumana Press
dc.publisher.countryUnited Statesen_GB
dc.publisher.countryYhdysvallat (USA)fi_FI
dc.publisher.country-codeUS
dc.publisher.isbn978-0-89603;978-1-58829;978-1-59259;978-1-59745;978-1-60327;978-1-60761;978-1-61737;978-1-61779;978-1-62703;978-1-934115;978-1-4939; 978-1-0716
dc.relation.doi10.1007/978-1-0716-3135-5_25
dc.relation.ispartofseriesMethods in Molecular Biology
dc.relation.volume2654
dc.source.identifierhttps://www.utupub.fi/handle/10024/193966
dc.titleIsolation of the B Cell Immune Synapse for Proteomic Analysis
dc.title.bookThe Immune Synapse
dc.year.issued2023

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