Discerning Autophagy Pathway Intermediates: Transmission Electron Microscopy Techniques

Tietueen suppeat tiedot
dc.contributor.authorBiazik, Joanna
dc.contributor.authorKallio, Katri
dc.contributor.authorGudmundsson, Sigurdur Runar
dc.contributor.authorEskelinen, Eeva-Liisa
dc.contributor.organizationfi=biolääketieteen laitos|en=Institute of Biomedicine|
dc.contributor.organization-code1.2.246.10.2458963.20.77952289591
dc.converis.publication-id499363537
dc.converis.urlhttps://research.utu.fi/converis/portal/Publication/499363537
dc.date.accessioned2026-01-21T12:36:08Z
dc.date.available2026-01-21T12:36:08Z
dc.description.abstract<p>In this chapter, you will learn guidelines on how to identify macroautophagy/autophagy pathway intermediates in transmission electron microscopy samples prepared using conventional techniques including chemical fixation and plastic embedding. We will describe the typical morphological features of phagophores, autophagosomes, amphisomes and autolysosomes induced by amino-acid starvation in mammalian cells. We will point out what you should keep in mind when interpreting transmission electron microscope images taken of very thin sections cut from much thicker cells and organelles. You will also learn how immuno-electron microscopy of endogenous MAP1 LC3/LC3 can be used to help identification of autophagy pathway intermediates.<br></p>
dc.embargo.lift2026-07-09
dc.format.pagerange29
dc.format.pagerange42
dc.identifier.eisbn978-3-031-88121-3
dc.identifier.isbn978-3-031-88120-6
dc.identifier.issn2509-6125
dc.identifier.olddbid212721
dc.identifier.oldhandle10024/195739
dc.identifier.urihttps://www.utupub.fi/handle/11111/53217
dc.identifier.urlhttps://link.springer.com/chapter/10.1007/978-3-031-88121-3_2
dc.identifier.urnURN:NBN:fi-fe202601217087
dc.language.isoen
dc.okm.affiliatedauthorEskelinen, Eeva-Liisa
dc.okm.discipline1182 Biochemistry, cell and molecular biologyen_GB
dc.okm.discipline3111 Biomedicineen_GB
dc.okm.discipline1182 Biokemia, solu- ja molekyylibiologiafi_FI
dc.okm.discipline3111 Biolääketieteetfi_FI
dc.okm.internationalcopublicationinternational co-publication
dc.okm.internationalityInternational publication
dc.okm.typeB2 Book
dc.publisherSpringer
dc.publisher.countrySwitzerlanden_GB
dc.publisher.countrySveitsifi_FI
dc.publisher.country-codeCH
dc.publisher.isbn978-81-322;978-3-540;978-3-642;978-3-662;978-3-7908;978-3-8274;978-3-8347;978-90-481;978-94-007;978-94-009;978-94-010;978-94-011;978-94-015;978-94-017;978-94-024;978-0-387;978-0-8176;978-1-4419;978-1-4612;978-1-4613;978-1-4614;978-1-4615;978-1-4684;978-1-4757;978-1-4899;978-1-4939;978-1-5041;978-3-319;978-1-4020;978-0-85729;978-1-4471;978-1-84628;978-1-84800;978-1-84882;978-1-84996;978-1-85233;978-3-211;978-3-7091;978-4-431;978-3-322;978-3-409;978-3-531;978-3-658;978-3-663;978-3-8100;978-981-287;978-981-10;978-981-13;978-3-030;978-981-32;978-981-15;978-981-16;978-981-329;978-981-334;978-981-336;978-3-031;978-981-19;
dc.relation.doi10.1007/978-3-031-88121-3_2
dc.relation.ispartofseriesLearning Materials in Biosciences
dc.source.identifierhttps://www.utupub.fi/handle/10024/195739
dc.titleDiscerning Autophagy Pathway Intermediates: Transmission Electron Microscopy Techniques
dc.title.bookAutophagy - From Molecular Mechanisms to Flux Control in Health and Disease
dc.year.issued2025

Tiedostot

Kokoelmat

Rinnakkaistallenteet