Aberrant glycosylation of α3 integrins as diagnostic markers in epithelial ovarian cancer

Abstract

<p><b>Background</b><br>Glycans are strongly involved in stability and function of integrins (ITG) and tetraspanin protein<br>CD63 and their respective interaction partners as they are dysregulated in the tumorigenic processes. Glycosylation changes is a universal phenomenon of cancer cells. In this study, glycosylation changes in epithelial ovarian cancer (EOC) are explored using tetraspanin and integrin molecules.</p><p><b>Methods</b><br>ITG and CD63 were immobilized from 10 EOC and 5 benign ovarian cyst fluid on microtiter wells and<br>traced with 3 glycan binding proteins (STn, WGA, UEA) conjugated on europium nanoparticles. Total protein measurements (ITG & CD63 immunoassays) were also performed. The most promising glycovariant candidates identified were then clinically evaluated on the whole cohort of 77 ovarian cyst fluids. Additional testing was performed in ascites fluid samples of liver cirrhosis (n = 2) and EOC (n = 4).</p><p><b>Results</b><br>Sialylated Tn antibody based glycovariants of ITGα3 (ITGα3STn) and CD63 (CD63STn) performed better<br>than corresponding protein epitope-based immunoassays, ITGα3IA and CD63IA respectively. Combined ITGα3 based assays (ITGα3IA + ITGα3STn) detected 49 out of 55 malignant & borderline cases without detecting any of the 22 benign and healthy cysts.</p><p><b>Conclusion</b><br>Our findings indicate the potential diagnostic application of ITGα3STn along with total ITGα3IA, which<br>could help reduce the unnecessary surgeries. The results encourage studying further the potential use of these novel assays to detect EOC at earlier clinical stages.</p>