Mass Spectrometry-based Serum Proteomics for Biomarker discovery and validation

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dc.contributor.authorSantosh D. Bhosale
dc.contributor.authorRobert Moulder
dc.contributor.authorPetri Kouvonen
dc.contributor.authorRiitta Lahesmaa
dc.contributor.authorDavid R. Goodlett
dc.contributor.organizationfi=Turun biotiedekeskus|en=Turku Bioscience Centre|
dc.contributor.organization-code1.2.246.10.2458963.20.18586209670
dc.contributor.organization-code2609200
dc.contributor.organization-code2609201
dc.converis.publication-id19257165
dc.converis.urlhttps://research.utu.fi/converis/portal/Publication/19257165
dc.date.accessioned2022-10-28T13:46:02Z
dc.date.available2022-10-28T13:46:02Z
dc.description.abstract<p> </p><p>Blood protein measurements are used frequently in the clinic in the assessment of patient health. Nevertheless, there remains the need for new biomarkers with better diagnostic specificities. With the advent of improved technology for bioanalysis and the growth of biobanks including collections from specific disease-risk cohorts, the plasma proteome has remained a target of proteomics research towards the characterization of disease related biomarkers. The following protocol presents a workflow for serum/plasma proteomics including details of sample preparation both with and without immunoaffinity depletion of the most abundant plasma proteins, and methodology for selected reaction monitoring mass spectrometry validation. </p>
dc.format.extent16
dc.format.pagerange451
dc.format.pagerange466
dc.identifier.isbn978-1-4939-7056-8
dc.identifier.issn1064-3745
dc.identifier.olddbid184175
dc.identifier.oldhandle10024/167269
dc.identifier.urihttps://www.utupub.fi/handle/11111/41646
dc.identifier.urlhttps://link.springer.com/protocol/10.1007/978-1-4939-7057-5_31
dc.identifier.urnURN:NBN:fi-fe2021042716671
dc.language.isoen
dc.okm.affiliatedauthorBhosale, Santosh
dc.okm.affiliatedauthorMoulder, Robert
dc.okm.affiliatedauthorKouvonen, Petri
dc.okm.affiliatedauthorLahesmaa, Riitta
dc.okm.affiliatedauthorGoodlett, David
dc.okm.discipline318 Medical biotechnologyen_GB
dc.okm.discipline318 Lääketieteen bioteknologiafi_FI
dc.okm.internationalcopublicationinternational co-publication
dc.okm.internationalityInternational publication
dc.okm.typeA3 Book
dc.publisherSpringer
dc.publisher.countryUnited Statesen_GB
dc.publisher.countryYhdysvallat (USA)fi_FI
dc.publisher.country-codeUS
dc.publisher.isbn978-81-322;978-3-540;978-3-642;978-3-662;978-3-7908;978-3-8274;978-3-8347;978-90-481;978-94-007;978-94-009;978-94-010;978-94-011;978-94-015;978-94-017;978-94-024;978-0-387;978-0-8176;978-1-4419;978-1-4612;978-1-4613;978-1-4614;978-1-4615;978-1-4684;978-1-4757;978-1-4899;978-1-4939;978-1-5041;978-3-319;978-1-4020;978-0-85729;978-1-4471;978-1-84628;978-1-84800;978-1-84882;978-1-84996;978-1-85233;978-3-211;978-3-7091;978-4-431;978-3-322;978-3-409;978-3-531;978-3-658;978-3-663;978-3-8100;978-981-287;978-981-10;978-981-13;978-3-030;978-981-32;978-981-15;978-981-16;978-981-329;978-981-334;978-981-336;978-3-031;978-981-19;
dc.relation.doi10.1007/978-1-4939-7057-5_31
dc.relation.ispartofseriesSerum/Plasma Proteomics
dc.relation.volume1619
dc.source.identifierhttps://www.utupub.fi/handle/10024/167269
dc.titleMass Spectrometry-based Serum Proteomics for Biomarker discovery and validation
dc.title.bookSerum/plasma proteomics : Methods and Protocols
dc.year.issued2017

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