Synthesis of bioconjugatable furimazine analogues

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Bioluminescence resonance energy transfer (BRET) has extensive applications in the studies of dynamic processes in various biological systems such as protein-protein interactions, signaling events, metabolites and drug concentrations. Recently it was found that BRET can not only act as a reporter of an event, but an effector which turns on functions in livings systems. Herein, we propose the synthesis of furimazine analogues, which are common substrates for luciferases in BRET studies, with a novel modification allowing the bioconjugation of small-molecules.

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