Expression of human collagenase-3 (MMP-13) by fetal skin fibroblasts is induced by transforming growth factor beta via p38 mitogen-activated protein kinase.
Penttinen R.; Kähäri V.; Crombleholme T.; Han J.; Ravanti L.; Foschi M.; Toriseva M.
Expression of human collagenase-3 (MMP-13) by fetal skin fibroblasts is induced by transforming growth factor beta via p38 mitogen-activated protein kinase.
Penttinen R.
Kähäri V.
Crombleholme T.
Han J.
Ravanti L.
Foschi M.
Toriseva M.
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042714999
Human collagenase-3 (MMP-13) is characterized by wide substrate specificity and limited tissue
specific expression. We have previously noted that human collagenase-3 (MMP-13) is expressed
by gingival fibroblasts in culture and during gingival wound repair characterized by minimal
scarring. Here we show that human MMP-13 is expressed by dermal fibroblasts during early
wound repair in fetal skin grafted on SCID mice. The expression of MMP-13 by fetal skin
fibroblasts in monolayer culture was enhanced by transforming growth factor
β
1 (TGF-
β
1) and
TGF-
β
3, whereas MMP-13 expression was not detected in neonatal skin fibroblasts. Treatment
of fetal skin fibroblasts with TGF-
β
1 potently activated p38 mitogen-activated protein kinase
(MAPK). Induction of MMP-13 expression by TGF-
β
1 was blocked by p38 MAPK inhibitor
SB203580, and by adenovirally delivered dominant negative form of p38
α
. These observations
demonstrate a remarkable difference in the regulation of collagenolytic capacity between fetal
and neonatal skin fibroblasts, which suggests a role for MMP-13 in rapid turnover of
collagenous matrix during repair of fetal cutaneous wounds, which heal without scar.
https://urn.fi/URN:NBN:fi-fe2021042714999
Tiivistelmä
Human collagenase-3 (MMP-13) is characterized by wide substrate specificity and limited tissue
specific expression. We have previously noted that human collagenase-3 (MMP-13) is expressed
by gingival fibroblasts in culture and during gingival wound repair characterized by minimal
scarring. Here we show that human MMP-13 is expressed by dermal fibroblasts during early
wound repair in fetal skin grafted on SCID mice. The expression of MMP-13 by fetal skin
fibroblasts in monolayer culture was enhanced by transforming growth factor
β
1 (TGF-
β
1) and
TGF-
β
3, whereas MMP-13 expression was not detected in neonatal skin fibroblasts. Treatment
of fetal skin fibroblasts with TGF-
β
1 potently activated p38 mitogen-activated protein kinase
(MAPK). Induction of MMP-13 expression by TGF-
β
1 was blocked by p38 MAPK inhibitor
SB203580, and by adenovirally delivered dominant negative form of p38
α
. These observations
demonstrate a remarkable difference in the regulation of collagenolytic capacity between fetal
and neonatal skin fibroblasts, which suggests a role for MMP-13 in rapid turnover of
collagenous matrix during repair of fetal cutaneous wounds, which heal without scar.
Kokoelmat
- Rinnakkaistallenteet [19207]