Isolation of the B Cell Immune Synapse for Proteomic Analysis
Humana Press
Pysyvä osoite
Verkkojulkaisu
Tiivistelmä
Recent technical developments have fueled increasing utilization of proteomics to gain new insights into various aspects of cellular behavior. In this chapter, we describe a method to specifically isolate immune synapses from mouse primary B cells. The method utilizes antibody-coated magnetic beads to induce the formation of the immune synapses and describes a protocol for the extraction of the cell-bead adhesions for mass spectrometry analysis. Finally, this method enables unveiling the large-scale protein content of the immune synapse.
Sarja
Methods in Molecular Biology